In principle most of the analytical techniques can be used to analyze lipids including fatty acids, phospholipids,  sphingolipids,  triglycerides and steroids. Routinely thin layer chromatography (TLC) was used for such purposes and is now replaced by faster technologies with better resolution.

• GC-MS for low molecular weight lipids of all different classes (<500 Da) see our GC-TOF technology platform using BinBase and SetupX we can routinely profile around 80 lipid compounds (out of 800) in one single run and semi-quantify those lipid-like substances contained in the FiehnLib mass spectral and retention index database with high reliability. This equals a coverage of 11% of the compounds (based on a 500 Da cut-off) in LipidMaps.

• LC-MS including HPLC-Chip/MS System, UPLC/MS, UPLC/FT-MS, LC-TOF/MS can be used to generate high resolution lipid profiles for lipids in the mass range 100-2000 Dalton. Using chromatography allows a better separation from plasma or tissue interferences, but also requires a longer run-time and handling of solvent systems.

Picture (CC-by) thanks to : Pietiläinen KH, Sysi-Aho M, Rissanen A, Seppänen-Laakso T, Yki-Järvinen H, Kaprio J, Oresic M. in Acquired Obesity Is Associated with Changes in the Serum Lipidomic Profile Independent of Genetic Effects – A Monozygotic Twin Study;PLoS ONE 2(2): e218. doi:10.1371/journal.pone.0000218

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• nano ESI-FTMS (chip based nano ESI FT-MS infusion) can be used for lipid fingerprinting, rapid classification, semi-quantification and structure elucidation of lipids. Using NanoMate chip based infusion together with high resolution FT-MS or Orbitrap technologies allows quick profiling (1 minute acquisition time) of lipids from crude extracts of plasma or tissue samples (plants, humans, bacteria, algea).

The largest curated lipid database LipidMaps contains a structure database with around 8000 unique structures (in SDF format which can be be viewed with the free Instant-JChem) which not only consist of the common free fatty acids, cholesterols, triglycerides and phospholipids, but 81 general lipid classes and 276 sub-categories. The LipidMaps consortium is based on a $35 million grant from the National Institute of General Medical Sciences (NIGMS) and supports more than 30 researchers at 18 universities. Recently researchers in Finland generated a lipid database (LipidDB) of roughly one million lipids using a scaffold library approach. Using a enumeration approach the LipidMonsterDB (lipidmaps.org) is even larger covering several million lipids.  

• LipidMaps - Largest curated lipid database, containig mass spectra, classifications, protocols of 10,000 lipid species
  
• LipidBank - a Japanese project containing 6000 lipid species, partly with experimental information
• CyberLipids - collection of links and infos
• SphingoMap - pathway map for sphingolipid biosynthesis
• LMSAD - Lipid Mass Spectrum Analysis Database (database of sphingolipid mass spectra in XML+viewer)
  
• Human Metabolome DB - can be used to query for lipid species (taxonomy class lipids)
• LipidMonsterDB - is a virtual database consisting of several million lipids which can be created with lipidmapstools (thanks to Manish Sud and Eoin Fahy).
  

Chemical standards of lipids are needed for quantification purposes and as internal standards. Synthetic lipids can be used as marker substances because they are not existing in living organisms.

• www.larodan.se - pure chemical lipid standards
• www.cayman.com - pure chemical lipid standards
• www.lipomics.com - now Tethys Bioscience
  
• www.avantilipids.com - Avanti Polar - some with Lipidmaps ID
  
• www.echelon-inc.com - pure chemical lipid standards
• www.sial.com - special collection on lipids in cell signalling (Sphingolipids, Phospholipids, Inositol Phosphates, Diacylglycerols)
• www.matreya.com - sphingolipids, FAMES, glycerolipids, glycerophospholipids, tocopherols
• www.funakoshi.com - Phospholipid Kit, Natural Phospholipids, Natural Sphingolipids [PDF]
  

Programs for qualifying and quantifying lipids became important with the recent development of LipidOmics and the requirement to analyze the lipidome (hence all lipids). Those programs can help during the target driven setup of precursor ion scans, neutral loss scans, data dependent scans  for  mass spectral  acquisition. Furthermore they contain features to  utilize the list of 10,000 known lipids and  perform data processing steps including extraction of MS/MS scans, clustering of lipids with similar MS/MS pattern and integration of monitored peaks. Please see also the other tools for comprehensive mass spectral analysis.

Lipid MS Predictor  v1.5 (Eoin Fahy)

• LipidMaps MS Tools - calculation of m/z values, formulas, sn1 and sn2 side chains and sn3 head groups
  
• LipidInspector - software for lipid analysis (free beta-version available) (see DOI)
• LipidQA (Washington University Biomedical Mass Spectrometry) [Download]
  
• LipidProfiler (MDS Sciex) - the Lipid Profiler software uses data from the QqTOF-MS or QTRAP-MS

• LIMSA - free software for lipid analysis from University of Helsinki (see DOI)
  
• FAAT - software for lipid analysis using FT-MS free download here
• LipidSearch - Department of Metabolome, Graduate School of Medicine, the University of Tokyo.
• Online Lipid Calculator - Department of Pharmacology, University of Colorado Denver
• ALEX - Analysis of Lipid EXperiments (ALEX)
  
• TriglyAPCI - tool for interpreting atmospheric pressure chemical ionization (APCI) mass spectra of triacylglycerols (TAGs) from the Institute of Organic Chemistry and Biochemistry, Prague, Czech Republic
• LipidX - for Data Dependent Acquisition (DDA) using the Molecular Fragmentation Query Language (MFQL) in shotgun lipidomics (mpi-cbg Dresden)
• AMDMS-SL (Automated Lipid Identification and Quantification by Multi-Dimensional Mass Spectrometry based Shotgun Lipidomics) (free download)
  

• Lipid Analysis Methods - Book Chapters [PDF, 230 pages] [Methods in Enzymology Elsevier]
• Lipid Analysis with Andrej Shevchenko - Advion seminar
  (Lipid Analysis by Nanospray Chip-Based Mass Spectrometry; Andrej Shevchenko MPI-CBG Dresden
• LipidLibrary - contains a comprehensive set of literature and reviews, analytical techniques, including mass spectra, NMR techniques, Ag+ chromatography
• Multiplexed Lipid Arrays of Anti-Immunoglobulin M-Induced Changes in the Glycerophospholipid Composition of WEHI-231 Cells [PDF] by Stephen B. Milne, Jeffrey S. Forrester, Pavlina T. Ivanova, Michelle D. Armstrong, and H. Alex Brown the independent XLS sheets can be obtained from http://www.signaling-gateway.org/data/lipid
• Molecular characterization of the lipidome by mass spectrometry; Christer Stenby Ejsing;
  Dissertation 2007;Technische Universität Dresden [PDF]
• Schistosomal lysophosphatidylserine: an immunomodulatory factor; Kim Retra
  Proefschrift 2007; Utrecht University; ISBN 978-90-393-4571-9; [PDF] [RCM]
  
• Characterization of Molecular Glycerophospholipids by Quadrupole Time-of-Flight Mass Spectrometry: Kim Ekroos; Dissertation TU Dresden 2003; [PDF]
• An automated workflow for rapid alignment and identification of lipid biomarkers obtained from chip-based direct infusion nanoelectrospray tandem mass spectrometry (ASMS 2008) [PDF]
• Algal and bacterial lipids - Research Seminar Tobias Kind [PPT]
  

• LipidMaps DB 2008 as information enhanced EXCEL file (19 MB ZIP) [XLS] [small XLS]
  
• List of 41,510 Glycerophospholipids (GPA, GPCho, GPEtn, GPGro, GPIns, GPInsP, GPSer) in
  an information enhanced EXCEL file (27 MB ZIP) [XLS]
• Oxy-lipids under investigation at WHNRC John Newman's group (DiHOME, DiHET, HEPE, HETE, HODE, HOTE, EpODE, EpOME, oxo-ETE, PGF1a, PGE1, PGE2, PGE3) [DOC]
• List of accurate masses from fatty acids for mass spectrometric analysis (MS and MS/MS)
  from C1 to C50 and double bonds n1 to n9 and general fatty acyls and alkyls [XLS] [HTML]
• A list of 444,080 monoacylglycerols, diacylglycerols and mostly triacylglycerols in a file called glycero-lipids-monster-db (168 MByte) can be found on the MAG-DAG-TAG site [LINK]