Department of Pharmacology and PharmacoGenomics Research Center

Inje University College of Medicine

Korea

Phone; 82-51-890-6412     Fax; 82-51-893-1232

                         E-mail; dstlkh@inje.ac.kr

EDUCATION

B. Sc. in Agriculture (Student Number: 86505-014)

Department of Agricultural Chemistry,

College of Agriculture and Life Sciences,

Seoul National University (1986-1990), Korea

M. Sc. in Agriculture (Student Number: 90505-508)

Department of Agricultural Chemistry,

Graduate School of Seoul National University (1990-1992), Korea

Ph. D. in Agriculture (Student Number: 99505-803)

School of Agricultural Biotechnology,

Graduate School of Seoul National University (1999-2002), Korea

OCCUPATION

1991 – 1998    Researcher, Analytical Research Team, Doosan Technical Center

1999 – 2000    Teaching Assistant, School of Applied Biology and Chemistry,

College of Agriculture and Life Sciences, Seoul National University

2002 – 2002    Post-doctoral Researcher, School of Agricultural Biotechnology,

Seoul National University

2003 – present     Assistant Professor, Dept. of Pharmacology and PharmacoGenomics Research Center, Inje University College of Medicine

2007 – present Vice director, Biomarker Research Center, Inje University

TRAINING

GC/MS Operation, Hewlett-Packard Analytical Education Center, 11-15 Oct. 1993, Atlanta, USA

DISSERTATION TITLE

M. Sc.: A Quantitative Analysis of Glyceollin II in Etiolated Soybean Seedlings.

       (Advisor: Prof. Chang-Kyu Park, Dept. of Agricultural Chemistry,

               Seoul National University)

Ph. D.: Metabolism and Pharmacokinetics of a Herbicide Pyribenzoxim and an    Insecticide Furathiocarb in Rats.

        (Advisor: Associate Prof. Jeong-Han Kim,

                 School of Agricultural Biotechnology, Seoul National University)

PUBLICATIONS

1.      Kim H and Liu KH, Screening of inhibitory effect on nine CYP isoforms by 20 herbal medications, Kor J Life Sci, 17, 334~339 (2007) March.

2.      Kim H, Seo KA, Lee HS, Lee CH, Shin JG and Liu KH, Enzyme kinetic study of a new cardioprotective agent, KR-32570 using human liver microsomes and recombinant CYP isoforms, Arch Pharm Res, 30, 469 ~ 474 (2007) April.

3.      Park KR, Lee JH, Choi CY, Liu KH, Seog DH, Kim YH, Kim DE, Yun CH, and Yea SS. Suppression of interleukin-2 gene expression by isoeugenol is mediated through down-regulation of NF-AT and NF-kB, Int Immunopharm, 7, 1251-1258 (2007) September.

4.      Shim JC, Shin JG, Kelly DL, Jung DU, Seo YS, Liu KH, Shon JH and Conley RR, Adjunctive treatment with a dopamine partial agonist, aripiprazole, for antipsychotic-induced hyperlactinemia: A placebo-controlled trial, Am J Psychiatry, Accepted for publication.

5.      Ryu JY, Song IS, Sunwoo YE, Shon JH, Liu KH, Cha IJ and Shin JG, Development of the “Inje Cocktail” for high-throughput evaluation of five human cytochrome P450 isoforms in vivo, Clin Pharm Ther, On-line publication (March 28, 2007).

6.      Yoon YJ, Kim KB, Kim H, Seo KA, Kim HS, Cha IJ, Liu KH, and Shin JG. Characterization of benidipine and its enantiomers metabolism by human liver cytochrome P450 enzymes, Drug Metab Dispos, On-line publication (May 30. 2007).

7.      Deng JW, Kim KB, Song IS, Zhou HH, Liu KH, and Shin JG. Determination of two HMGCoA reductase inhibitors, pravastatin and pitavastatin in plasma samples using liquid chromatography-tandem mass spectrometry for pharmaceutical study, Biomed Chromatogr. Accepted for publication (2007. 6. 25).

8.      Lee SJ, Lee SS, Jeong HE, Shon JH, Ryu JY, Sunwoo YE, Liu KH, Kang W, Park YJ, Shin CM, and Shin JG, The CYP3A4*18 allele, the most frequent coding variant in Asian populations, does not significantly affect the midazolam disposition in heterozygous individuals. Drug Metab Dispos, Accepted for publication (2007, 8. 17).

9.      Liu KH, Kim MJ, Jeon BH, Shon JH, Cha IJ, Cho KH, Lee SS and Shin JG, Inhibition of human cytochrome P450 isoforms and NADPH-CYP reductase in vitro by Epimedii herba. J Clinical Pharmacy Therapeutics, 31, 83-91 (2006). February.

10.  Liu KH, Moon JK, Seo JS, Park BS, Koo SJ, Lee HS, and Kim JH, Characterization of pyribenzoxim metabolizing enzymes in rat liver microsomes. J Toxicol Pub Health, 22, 1-8 (2006) February.

11.  Kim H, Kang S, Kim H, Yoon YJ, Cha EY, Lee HS, Kim JH, Yea SS, Lee SS, Shin JG, and Liu KH, In vitro metabolism of a new cardioprotective agent, KR-32570 in the human liver microsomes. Rapid Communication in Mass Spectrometry, 20, 837-843 (2006), March.

12.  Kim H, Yoon YJ, Shon JH, Cha IJ, Shin JG, and Liu KH, Inhibitory effects of fruit juices on cytochrome P450 3A (CYP3A) activity, Drug Metab Dispos, 34, 521-523 (2006), April.

13.  Choi H, Moon JK, Liu KH, Park HW, Ihm YB, Park BS, Kim JH, Risk assessment of human exposure to cypermethrin during treatment of mandarin fields, Arch Env Contam Toxicol, 50, 437-442 (2006), April.

14.  Park JY, Kim KA, Park PW, Lee OJ, Kang DK, Shon JH, Liu KH, and Shin JG, Effects of CYP3A5*3 genotype on the pharmacokinetics and pharmacodynamics of alprazolam in healthy subjects. Clin Pharmcol Ther, 79, 590-599 (2006), June.

15.  Lee HG, Moon JK, Choi H, Park HW, Chang CH, Park BS, Lee HS, Hwang EC, Lee YD, Liu KH*, Kim JH*, Stereoselective metabolism of endosulfan by human liver microsomes and human cytochrome P450 isoforms, Drug Metab Dispos, 34 1090-1095 (2006), July (* co-corresponding authors).

16.  Kang HJ, Lee SS, Lee CH, Shin JC, Shin HJ, Liu KH, Yoo MA, and Shin JG, Neurotoxic pyridinium metabolites of haloperidol are substrate of human organic cation transports (hOCTs). Drug Metab Dispos, 34 1145-1151 (2006), July.

17.  Ku HY, Kim H, Shon JH, and Liu KH. Inhibition of drug-metabolizing enzyme and drug transporter by major components of Phellodendri cortex. J Marine Bioscience and Biotechnology, 1 213-217 (2006), September.

18.  Kim H, Yoon YJ, Kim H, Kang S, Cheon HG, Yoo SE, Shin JG and Liu KH, Characterization of human liver cytochrome P450 enzymes involved in the metabolism of a new cardioprotective agent, KR-33028, Toxicol Lett, 166, 105-114 (2006) October

19.  Liu KH, Kim MK, Kim MJ, Shon JH, Desta Z, and Shin JG, Characterization of ebastine, hydroxyebastine, and carebastine metabolism by human liver microsomes and expressed P450 enzymes: major roles for CYP2J2 and CYP3A, Drug Metab Dispos, 34 1793-1797 (2006), November.

20.  Kang W, Lee DJ, Liu KH, Sunwoo YE, Kwon KI, Cha IJ, Shin JG. Analysis of benidipine enantiomers in human plasma by liquid chromatography-mass spectrometry using a macrocyclic antibiotic (Vancomycin) chiral stationary phase column. J Chromatogr B, 814, 75-81 (2005), January

21.  Liu KH, Kim MJ, Shon JH, Moon YS, Seol SY, Kang W, Cha IJ, Shin JG, Stereoselective inhibition of cytochrome P450 forms by lansoprazole and omeprazole in vitro. Xenobiotica, 35, 27-38 (2005), January

22.  Liu KH, Kim MJ, Jung WM, Kang W, Cha IJ, Shin JG, Lansoprazole enantiomer activates human liver microsomal CYP2C9 catalytic activity in a stereospecific and substrate-specific manner. Drug Metab Dispos, 33, 209-213 (2005), February

23.  Lee SS, Jeong HE, Liu KH, Ryu JY, Moon T, Yoon CN, Oh SJ, Yun CH, Shin JG. Identification and functional characterization of novel CYP2J2 variants: G312R variant causes loss of enzyme catalytic activity. Pharmacogenetics and Genomics, 15, 105-113 (2005), February

24.  Shon JH, Yoon YR, Kim MJ, Kim KA, Lim YC, Liu KH, Shin DH, Lee CH, Cha IJ, Shin JG. Chlorpropamide 2-hydroxylation is catalysed by CYP2C9 and CYP2C19 in vitro: chlorpropamide disposition is influenced by CYP2C9, but not by CYP2C19 genetic polymorphism. Br J Clin Pharmacol, 59, 552-563 (2005), May.

25.  Liu KH, Moon JK, Kang SH, Koo SJ, Lee HS and Kim JH, Identification of rat urinary and fecal metabolites of a new herbicide pyribenzoxim. J Agric Food Chem. 53, 6713-6717 (2005), September.

26.  Liu KH, Moon JK, Choi HS, Youn YG, Park BS, Lee HS, Kim JH, Photodegradation of bistrifluron in aqueous acetonitrile solution by UV irradiation. Bull Env Contam Toxicol, 75, 451-458 (2005). September.

27.  Kim MJ, Kim H, Cha IJ, Park JS, Shon JH, Shin JG and Liu KH, High-throughput screening of inhibitory potential of nine cytochrome P450 enzymes in vitro using liquid chromatography-tandem mass spectrometry. Rapid Communication in Mass Spectrometry, 19, 2651-2658 (2005). October.

28.  Kim H, Yoon YJ, Kim H, Cha EY, Lee HS, Kim JH, Yi KY, Lee SK, Cheon JG, Yoo SE, Lee SS, Shin JG and Liu KH, In vitro metabolism of a new cardioprotective agent, KR-33028 in the human liver microsomes and cryopreserved human hepatocytes. Arch Pharm Res, 28, 1287-1292 (2005). November.

29.  Lee JS, Kim DH, Liu KH, Oh TK and Lee CH, Identification of flavonoids using liquid chromatography with electrospray ionization and ion trap tandem mass spectrometry with an MS/MS library. Rapid Communication in Mass Spectrometry, 19, 3539-3548 (2005). December.

30.  Byoun JY, Choi H, Moon JK, Park HW, Liu KH, Ihm YB, Park BS and Kim JH, Risk assessment of human exposure to methidathion during harvest of cucumber in green house. J Toxicol Pub Health, 21, 297-301 (2005). December.

31.  Liu KH, Lee YK, Sunwoo YE, Yu KS, Kang W, Lee SS, Yoon YR and Shin JG. High-performance liquid chromatographic assay of zonisamide (1,2-benzisoxazole-3-methanesulfonamide) in human plasma using a solid-phase extraction technique. Chromatographia 59, 497-500 (2004), April.

32.  Liu KH, Lee YM, Shon JH, Kim MJ, Lee SS, Yoon YR, Cha IJ and Shin JG. The potential of pranlukast and zafirlukast to inhibit human liver cytochrome P450 enzymes. Xenobiotica 34, 429-438 (2004), May

33.  Kang W, Yun HY, Liu KH, Kwon KI, Shin JG. Determination of benidipine in human plasma using high-performance liquid chromatography-tandem mass spectrometry. J. Chromatogr. B. 805, 311-314 (2004), June.

34.  Shon JH, Kim TJ, Lee SS, Liu KH, Yoon YR, Cha IJ, Shin JG. Pharmacokinetics and pharmacodynamics of fexofenadine in relation to genetic polymorphism of MDR-1 C3435T/G2677T. Kor J Clin Pharmacol Ther. 12, 47-56, 2004, June.

35.  Yu KS, Cho JY, Jang IJ, Hong KS, Chung JY, Kim JR, Lim HS, Oh DS, Yi SY, Liu KH, Shin JG, Shin SG. Effect of the CYP3A5 genotype on the pharmacokinetics of intravenous midazolam during inhibited and induced metabolic states. Clin Pharmacol Ther. 76, 104-112 (2004) August

36.  Liu KH, Lee YK, Ryu JY, Lee DJ, Kang W, Lee SS, Yoon YR, Shin JG, Simple and sensitive assay of torasemide in human plasma by high-performance liquid chromatography using a monolithic silica column. Chromatographia, 60, 639-643 (2004) December

37.  Kang W, Liu KH, Ryu JY, Shin JG. Simultaneous determination of ebastine and its three metabolites in plasma using liquid chromatography-tandem mass spectrometry, J Chromatogr B, 813, 75-80 (2004), December

38.  Kim J, Liu KH, Kang SH, Koo SJ and Kim JH. Aerobic soil metabolism of a new herbicide, LGC-42153. J Agric Food Chem 51, 710-714 (2003), January

39.  Liu KH, Kim CS, and Kim JH. Human exposure assessment to mancozeb during treatment of mandarin fields. Bulletin of Environmental Contamination and Toxicology, 70, 336-342 (2003), February

40.  Park MK, Liu KH, Lim Y, Lee YH, Hur HG, and Kim JH. Biotransformation of a fungicide ethaboxam by soil fungus Cunninghamella elegans. J Microbiol Biotechnol 13 (1), 43-49 (2003), March

41.  Liu KH and Kim JH. In vitro dermal penetration study of carbofuran, carbosulfan, and furathiocarb. Arch Toxicol 77 (5), 255-260 (2003), May

42.  Moon JK, Park HW, Choi H, Hong YS, Liu KH, Lee YH, Lee KS and Kim JH. Residue pattern of fenitrothion in grapes. J Kor Soc Hort Sci 44, 497-502 (2003), August

43.  Kim J, Liu KH, Kang SH, Koo SJ, and Kim JH. Degradation of the sulfonylurea herbicide LGC-42153 in flooded soil. Pest Manag Sci 59, 1037-1042 (2003), September

44.  Kim KA, Kim MJ, Park JY, Shon JH, Yoon YR, Lee SS, Liu KH, Chun JH, Hyun MH, and Shin JG. Stereoselective metabolism of lansoprazole by human liver cytochrome P450 enzymes. Drug Metab Dispos 31, 1227-1234 (2003), October

45.  Liu KH, Sung HJ, Lee HK, Song BH, Ihm YB, Kim K, Lee HS and Kim JH. Dermal pharmacokinetics of the insecticide furathiocarb in Sprague-Dawley rats. Pest Management Science 58, 57-62 (2002)

46.  Seo JS, Liu KH, Chung KH, Shin JS, and Kim JH. Bioconcentration and Depuration of Pyribenzoxim in Common Carps (Cyprinus carpio). Bulletin of Environmental Contamination and Toxicology, 68, 617-622 (2002)

47.  Keum YS, Liu KH, Seo JS, Kim JH, Kim K, Kim YH, and Kim PJ. Dissipation of Foliar Residues of Diafenthiuron and Its Metabolites. Bulletin of Environmental Contamination and Toxicology, 68, 845-821 (2002)

48.  Park MK, Liu KH, Lee YH, Lee YS, Hur HG, and Kim JH. In vitro Metabolism of Ethaboxam by Rat Liver Microsomes. Agric Chem Biotechnol 45 (2), 94-98 (2002)

49.  Kim KY, Seo JS, Liu KH, and Kim JH. Determination of LogP of a New Insecticide Bistrifluron by HPLC. Agric Chem Biotechnol 45 (2), 99-100 (2002)

50.  Liu KH, Rhee S, and Kim JH. Structural Investigation of Pyribenzoxim Using X-ray Crystallography. Bulletin of the Korean Chemical Society, 23 (7), 1042-1044 (2002)

51.  Liu KH, and Kim JH. The skin penetration study of pesticide. AgroBiotech, 9 (2), 69-81 (2002), December

52.  Liu KH, Byoun JY, Sung HJ, Lee HK, Kim K, Lee HS, and Kim, JH. Simultaneous Determination of Furathiocarb and its Metabolites in Biological Tissues using High Performance Liquid Chromatography and Post-Column Derivatization, Chromatographia 53 (11/12), 687-690 (2001)

53.  Liu KH, Moon JK and Kim JH. Metabolic mass balance of pyribenzoxim in rats. Ann RCNBMA 10, 9-13 (2001)

54.  Liu KH, Moon JK, Sung HJ, Kang SH, Koo SJ, Lee HS, and Kim JH. In vivo pharmacokinetics of pyribenzoxim in rats. Pest Management Science 57, 1155-1160 (2001)

55.  Kim KY, Kim J, Liu KH, Lee HS, and Kim JH, In vitro Metabolism of Pyribenzoxim, Agric Chem Biotechnol 43 (1), 49-53 (2000)

56.  Keum YS, Liu KH, Lee YS, Lee JS, Chung BJ, and Kim JH. Gas Chromatographic Analysis of Methiocarb and its Metabolites in Soil and Rice, Chromatographia 52 (3/4), 237-241 (2000)

RESEARCH EXPERIENCES

1. Structure identification of drug metabolites and plant secondary metabolites using tandem mass spectrometry and NMR.

2. Instrumental analysis technique: HPLC (Agilent, Waters, Gilson, Varian etc), GC (Agilent, Varian etc.), GC-MS (Agilent, Shimadzu etc.), LC-MS/MS (ABI 3000, QTrap 4000, Micromass Premier XE, Agilent 6410 etc.), NMR (Brucker, Varian etc.)

3. Endogeneous metabolite profiling in biological samples such as plasma and urine: Eicosanoids, Steroids, Bile acids, and Tryptophan metabolites

4. Animal experiments: Pharmacokinetic study (Absorption, Distribution, Metabolism, and Excretion)

5. Enzyme kinetic study using microsomes, rCYP isoforms, and rUGT isoforms.

6. Drug metabolism study: Metabolite identification, Identification of metabolizing enzyme (CYP isoforms, UGT isoforms etc.) responsible for drug metabolism, Kinetics.

7. Drug interaction study: CYP inhibition study using liver microsomes, P-glycoprotein inhibition study using cell line system, Induction study using Caco-2 and LS-174T cells.