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Team Leader / Project Scientist

UC Davis Genome Center - Metabolomics
GBSF building room 1212A
451 East Health Science Drive

Davis

California

95616

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Research Interest

  • Liquid chromatography–mass spectrometry (LC–MS) and gas chromatography–mass spectrometry-based (GC–MS) metabolomics and lipidomics; GC–MS and GCxGC–MS volatilomics
  • Novel technologies for MS-based metabolomics
  • Method development and validation
  • Interpretation of chromatographic and MS data
  • Pitfalls and troubleshooting in metabolomics/lipidomics
  • Nutritional and food metabolomics/lipidomics
  • Data processing software in metabolomics/lipidomics
  • Statistical data analysis (multivariate data analysis, network mapping)
  • Target analysis of food contaminants and biologically active compounds

Education

6/2010: Habilitation in Food Chemistry and Analysis, Institute of Chemical Technology, Prague, Czech Republic

7/2002–2/2009: PhD study in Food Chemistry and Analysis, Institute of Chemical Technology, Prague, Czech Republic

9/1997–6/2002: MSc study in Food Chemistry and Analysis, Institute of Chemical Technology, Prague, Czech Republic

Past Positions

10/2012–6/2015: Associate Specialist, UC Davis Genome Center, Metabolomics Fiehn Lab, University of California, Davis, CA, USA

6/2010–8/2012: Full-time Associate Professor, Institute of Chemical Technology, Prague, Faculty of Food and Biochemical Technology, Department of Food Analysis and Nutrition (Department of Food Chemistry and Analysis), Prague, Czech Republic

4/2009–5/2010: Assistant Professor, Institute of Chemical Technology, Prague, Czech Republic

9/2005–3/2009: Assistant, Institute of Chemical Technology, Prague, Czech Republic

4/2004–8/2012: Analyst, Metrological and Testing Laboratory of the Institute of Chemical Technology, Prague, Czech Republic

8–9/2004: Visiting Scientist, United States Department of Agriculture, Agriculture Research Service, Eastern Regional Research Center, Wyndmoor, PA, USA

8/2001: Visiting Scientist, The Netherlands Institute for Fisheries Research, IJmuiden, The Netherlands

Awards

  • 2007: The Ioannes Marcus Marci Spectroscopic Society: The Young Authors’ Best Paper in Spectroscopy (Gas Chromatography–Time-of-Flight Mass Spectrometry: An Effective Analytical Tool in the Analysis of Complex Food and Environmental Matrices)

Publications

Journal papers

(41) H. Tsugawa, T. Cajka, T. Kind, Y. Ma, B. Higgins, K. Ikeda, M. Kanazawa, J. VanderGheynst, O. Fiehn, M. Arita: MS-DIAL: data-independent MS/MS deconvolution for comprehensive metabolome analysis. Nature Methods 12 (2015) 523–526. (doi: 10.1038/nmeth.3393)

(40) T. Cajka, O. Fiehn: Comprehensive analysis of lipids in biological systems by liquid chromatography–mass spectrometry. TrAC-Trends in Analytical Chemistry 61 (2014) 192–206. (doi: 10.1016/j.trac.2014.04.017)

(39) T. Cajka, M. Vaclavikova, Z. Dzuman, L. Vaclavik, J. Ovesna, J. Hajslova: Rapid LC–MS-based metabolomics method to study Fusarium infection of barley, Journal of Separation Science 37 (2014) 912–919. (doi: 10.1002/jssc.201301292)

(38) K. Kalachova, J. Pulkrabova, T. Cajka, L. Drabova, M. Stupak, J. Hajslova: Gas chromatography–triple quadrupole tandem mass spectrometry: A powerful tool for the (ultra)trace analysis of multi-class environmental contaminants in fish and fish feed. Analytical and Bioanalytical Chemistry 405 (2013) 7803–7815 (doi: 10.1007/s00216-013-7000-4)

(37) T. Cajka, H. Danhelova, A. Vavrecka, K. Riddellova, V. Kocourek, F. Vacha, J. Hajslova: Evaluation of direct analysis in real time ionization–mass spectrometry (DART–MS) in fish metabolomics aimed to assess the response to dietary supplementation. Talanta 115 (2013) 263-270. (doi: 10.1016/j.talanta.2013.04.25)

(36) T. Cajka, H. Danhelova, M. Zachariasova, K. Riddellova, J. Hajslova: Application of direct analysis in real time ionization–mass spectrometry (DART–MS) in chicken meat metabolomics aiming at the retrospective control of feed fraud. Metabolomics 9 (2013) 545–557. (doi: 10.1007/s11306-013-0495-z)

(35) K. Kalachova, T. Cajka, C. Sandy, J. Hajslova, J. Pulkrabova: High throughput sample preparation in combination with gas chromatography coupled to triple quadrupole tandem mass spectrometry (GC–MS/MS): A smart procedure for (ultra)trace analysis of brominated flame retardants in fish. Talanta 105 (2013) 109–116. (doi: 10.1016/j.talanta.2012.11.073)

(34) O. Lacina, M. Zachariasova, J. Urbanova, M. Vaclavikova, T. Cajka, J. Hajslova: Critical assessment of extraction methods for the simultaneous determination of pesticide residues and mycotoxins in fruits, cereals, spices and oil seeds employing ultra-high performance liquid chromatography–tandem mass spectrometry.Journal of Chromatography A 1262 (2012) 8–18. (doi: 10.1016/j.chroma.2012.08.097)

(33) L. Vaclavik, A. Schreiber, O. Lacina, T. Cajka, J. Hajslova: Liquid chromatography–mass spectrometry-based metabolomics for authenticity assessment of fruit juices. Metabolomics 8 (2012) 793–803. (doi: 10.1007/s11306-011-0371-7)

(32) T. Cajka, C. Sandy, V. Bachanova, L. Drabova, K. Kalachova, J. Pulkrabova, J. Hajslova: Streamlining sample preparation and gas chromatography-tandem mass spectrometry analysis of multiple pesticide residues in tea. Analytica Chimica Acta 743 (2012) 51–60. (doi: 10.1016/j.aca.2012.06.051)

(31) K. Kalachova, J. Pulkrabova, T. Cajka, L. Drabova, J. Hajslova: Implementation of comprehensive two-dimensional gas chromatography–time-of-flight mass spectrometry for the simultaneous determination of halogenated contaminants and polycyclic aromatic hydrocarbons in fish. Analytical and Bioanalytical Chemistry403 (2012) 2813–2824. (doi: 10.1007/s00216-012-6095-3)

(30) H. Danhelova, J. Hradecky, S. Prinosilova, T. Cajka, K. Riddellova, L. Vaclavik, J. Hajslova: Rapid analysis of caffeine in various coffee samples employing direct analysis in real time ionization–high-resolution mass spectrometry. Analytical and Bioanalytical Chemistry 403 (2012) 2883–2889. (doi: 10.1007/s00216-012-5820-2)

(29) J. Lojza, T. Cajka, V. Schulzova, K. Riddellova, J. Hajslova: Analysis of isoflavones in soybeans employing direct analysis in real time ionization–high-resolution mass spectrometry. Journal of Separation Science 35(2012) 476–481. (doi: 10.1002/jssc.201100882)

(28) K. Kalachova, J. Pulkrabova, L. Drabova, T. Cajka, V. Kocourek, J. Hajslova: Simplified and rapid determination of polychlorinated biphenyls, polybrominated diphenyl ethers, and polycyclic aromatic hydrocarbons in fish and shrimps integrated into a single method. Analytica Chimica Acta 707 (2011) 84–91. (doi: 10.1016/j.aca.2011.09.016)

(27) T. Cajka, K. Riddellova, P. Zomer, H. Mol, J. Hajslova: Direct analysis of dithiocarbamate fungicides in fruit by ambient mass spectrometry. Food Additives & Contaminants: Part A: Chemistry, Analysis, Control, Exposure & Risk Assessment 28(10) (2011) 1372–1382 (in a special issue on Applications of High Resolution Mass Spectrometry to Food Safety). (doi: 10.1080/19440049.2011.590456)

(26) T. Cajka, K. Riddellova, M. Tomaniova, J. Hajslova: Ambient mass spectrometry employing a DART ion source for metabolomic fingerprinting/profiling: A powerful tool for beer origin recognition. Metabolomics 7(4)(2011) 500–508. (doi: 10.1007/s11306-010-0266-z)

(25) L. Vaclavik, V. Hrbek, T. Cajka, B.-A. Rohlik, P. Pipek, J. Hajslova: Authentication of animal fats using direct analysis in real time (DART) ionization–mass spectrometry and chemometric tools. Journal of Agricultural and Food Chemistry 59 (2011) 5919–5926. (doi: 10.1021/jf200734x)

(24) J. Hajslova, T. Cajka, L. Vaclavik: Challenging applications offered by direct analysis in real time (DART) in food-quality and safety analysis. TrAC-Trends in Analytical Chemistry 30 (2011) 204–218. (doi: 10.1016/j.trac.2010.11.001)

(23) M. Zachariasova, T. Cajka, M. Godula, A. Malachova, Z. Veprikova, J. Hajslova: Analysis of multiple mycotoxins in beer employing (ultra)-high resolution mass spectrometry. Rapid Communications in Mass Spectrometry 24 (2010) 3357–3367. (doi: 10.1002/rcm.4746)

(22) T. Cajka, K. Riddellova, M. Tomaniova, J. Hajslova: Recognition of beer brand based on multivariate analysis of volatile fingerprint. Journal of Chromatography A 1217 (2010) 4195–4203. (doi: 10.1016/j.chroma.2009.12.049)

(21) T. Cajka, K. Riddellova, E. Klimankova, M. Cerna, F. Pudil, J. Hajslova: Traceability of olive oil based on volatiles pattern and multivariate analysis. Food Chemistry 121 (2010) 282–289. (doi: 10.1016/j.foodchem.2009.12.011)

(20) J. Pulkrabova, K. Kalachova, L. Drabova, T. Cajka, J. Poustka, J. Hajslova: Rapid method for simultaneous determination of PCBs, PBDEs and PAHs in fish samples. Organohalogen Compounds 71 (2009) 2230–2235.

(19) I. Stanimirova, B. Üstün, T. Cajka, K. Riddellova, J. Hajslova, L.M.C. Buydens, B. Walczak: Tracing the geographical origin of honeys based on volatile compounds profiles assessment using pattern recognition techniques. Food Chemistry 188(1) (2010) 171–176. (doi: 10.1016/j.foodchem.2009.04.079)

(18) L. Vaclavik, T. Cajka, V. Hrbek, J. Hajslova: Ambient mass spectrometry employing direct analysis in real time (DART) ion source for olive oil quality and authenticity assessment. Analytica Chimica Acta 645(1–2)(2009) 56–63. (doi: 10.1016/j.aca.2009.04.043)

(17) L. Lojzova, K. Riddellova, J. Zrostlikova, J. Schurek, T. Cajka: Alternative GC-MS approaches in the analysis of substituted pyrazines and other volatile aromatic compounds formed during Maillard reaction in potato chips. Analytica Chimica Acta 641(1–2) (2009) 101–109. (doi:10.1016/j.aca.2009.03.036)

(16) T. Cajka, J. Hajslova, F. Pudil, K. Riddellova: Traceability of honey origin based on volatiles pattern processing by artificial neural networks. Journal of Chromatography A 1216(9) (2009) 1458–1462. (doi: 10.1016/j.chroma.2008.12.066)

(15) J. Hajslova, L. Vaclavik, J. Pulkrabova, J. Poustka, T. Cajka: DART–TOFMS: A challenging approach in rapid monitoring of brominated flame retardants in environmental matrices. Organohalogen Compounds 70(2008) 922–925.

(14) T. Cajka, L. Vaclavik, K. Riddellova, J. Hajslova: GC–TOF-MS and DART–TOF-MS: Challenges in the analysis of soft drinks. LC GC Europe 21(5) (2008) 250–256. (WEB)

(13) T. Cajka, J. Hajslova, O. Lacina, K. Mastovska, S.J. Lehotay: Rapid analysis of multiple pesticide residues in fruit-based baby food using programmed temperature vaporiser injection–low-pressure gas chromatography–high-resolution time-of-flight mass spectrometry. Journal of Chromatography A 1186(1–2) (2008) 281–294 (in a special issue on Trends and Developments in Gas Chromatography). (doi:10.1016/j.chroma.2007.12.009)

(12) E. Klimánková, K. Holadová, J. Hajšlová, T. Cajka, J. Poustka, M. Koudela: Aroma profiles of five basil (Ocimum basilicum L.) cultivars grown under conventional and organic conditions. Food Chemistry 107(1)(2008) 464–472. (doi: 10.1016/j.foodchem.2007.07.062)

(11) J. Hajšlová, J. Pulkrabová, J. Poustka, T. Cajka, T. Randák: Brominated flame retardants and related chlorinated persistent organic pollutants in fish from river Elbe and its main tributary Vltava.Chemosphere 69(8) (2007) 1195–1203. (doi: 10.1016/j.chemosphere.2007.06.030)

(10) T. Cajka, J. Hajšlová, J. Cochran, K. Holadová, E. Klimánková: Solid phase microextraction–comprehensive two-dimensional gas chromatography–time-of-flight mass spectrometry for the analysis of honey volatiles. Journal of Separation Science 30(4) (2007) 534–546 (in a special issue on Separation Science in Food Analysis). (doi: 10.1002/jssc.200600413)

(9) T. Cajka, J. Hajšlová: Gas chromatography–time-of-flight mass spectrometry in food analysis. LC GC Europe, 20(1) (2007) 25–26, 28–31. (WEB)

(8) L. Dunovská, T. Cajka, J. Hajšlová, K. Holadová: Direct determination of acrylamide in food by gas chromatography–high-resolution time-of-flight mass spectrometry. Analytica Chimica Acta 578(2) (2006) 234–240. (doi: 10.1016/j.aca.2006.07.001)

(7) T. Randák, V. Žlábek, J. Kolárová, Z. Svobodová, J. Hajšlová, Z. Široká, M. Jánská, J. Pulkrabová, T. Cajka, J. Jarkovský: Biomarkers detected in chub (Leuciscus cephalus L.) to evaluate contamination of the Elbe and Vltava rivers, Czech Republic. Bulletin of Environmental Contamination and Toxicology 76(2) (2006) 233–241. (doi: 10.1007/s00128-006-0912-3)

(6) T. Cajka, K. Maštovská, S.J. Lehotay, J. Hajšlová: Use of automated direct sample introduction with analyte protectants in the GC–MS analysis of pesticide residues. Journal of Separation Science 28(9–10)(2005) 1048–1060 (in a special issue on Food Analysis). (doi: 10.1002/jssc.200500050)

(5) T. Cajka, J. Hajšlová, R. Kazda, J. Poustka: Challenges of gas chromatography–high-resolution time-of-flight mass spectrometry for simultaneous analysis of polybrominated diphenyl ethers and other halogenated persistent organic pollutants in environmental samples. Journal of Separation Science 28(7) (2005) 601–611. (doi: 10.1002/jssc.200401850)

(4) R. Kazda, J. Hajšlová, J. Poustka, T. Cajka: Determination of polybrominated diphenyl ethers in human milk samples in the Czech Republic Comparative study of negative chemical ionisation mass spectrometry and time-of-flight high-resolution mass spectrometry. Analytica Chimica Acta 520 (2004) 237–243. (doi:10.1016/j.aca.2004.04.069)

(3) T. Cajka, J. Hajšlová: Gas chromatography–high-resolution time-of-flight mass spectrometry in pesticide residue analysis: advantages and limitations. Journal of Chromatography A 1058 (2004) 251–261 (in a special issue on Mass Spectrometry: Innovation and Application). (doi: 10.1016/j.chroma.2004.07.097)

(2) J. Zrostlíková, J. Hajšlová, T. Cajka: Evaluation of two-dimensional gas chromatography–time-of-flight mass spectrometry for the determination of multiple pesticide residues in fruit. Journal of Chromatography A1019 (2003) 173–186. (doi: 10.1016/S0021-9673(03)01302-5)

(1) T. Cajka, J. Hajšlová: Polychlorinated biphenyls and organochlorine pesticides in human milk from locality Prague: A comparative study. Bulletin of Environmental Contamination and Toxicology 70(5) (2003) 913–919. (doi: 10.1007/s00128-003-0069-2)

Book chapters

(9) L. Vaclavik, T. Cajka, W. Zhou, P.G. Wang: Survey of mass spectrometry-based high-throughput methods in food analysis. In: High Throughput Analysis for Food Safety. P.G. Wang, M.F. Vitha, and J.F. Kay (Eds.), ISBN: 978-1-118-39630-8, Wiley (2014) pp. 15–72. (doi: 10.1002/9781118907924.ch02)

(8) M. Vaclavikova, L. Vaclavik, T. Cajka: High-throughput analysis of mycotoxins. In: High Throughput Analysis for Food Safety. P.G. Wang, M.F. Vitha, and J.F. Kay (Eds.), ISBN: 978-1-118-39630-8, Wiley (2014) pp. 231–266. (doi: 10.1002/9781118907924.ch08)

(7) T. Cajka: Gas chromatography–time-of-flight mass spectrometry in food and environmental analysis. In:Comprehensive Analytical Chemistry, Volume 61: Advanced Techniques in Gas Chromatography–Mass Spectrometry (GC–MS–MS and GC–TOF–MS) for Environmental Chemistry. I. Ferrer and E. Thurman (Eds.), ISBN: 978-0-444-62623-3, Elsevier (2013) pp. 271–302. (doi: 10.1016/B978-0-444-62623-3.00012-5)

(6) T. Cajka, J. Hajslova: Volatile compounds in food authenticity and traceability testing. In: Food Flavors: Chemical, Sensory and Technological Properties. H. Jelen (Ed.), ISBN: 9781439814918, CRC Press (2011) pp. 355–411.

(5) T. Cajka, J. Hajslova: Halogenated persistent organic pollutants and polycyclic aromatic hydrocarbons in food. In: Mass Spectrometry in Food Safety: Methods and Protocols. J. Zweigenbaum (Ed.), ISBN: 978-1617791352, Humana Press (2011) pp. 373–410. (doi: 10.1007/978-1-61779-136-9_15)

(4) J. Hajslova, M. Zachariasova, T. Cajka: Analysis of multiple mycotoxins in food. In: Mass Spectrometry in Food Safety: Methods and Protocols. J. Zweigenbaum (Ed.), ISBN: 978-1617791352, Humana Press (2011) pp. 233–258. (doi: 10.1007/978-1-61779-136-9_10)

(3) T. Cajka, J. Hajslova, K. Mastovska: Mass spectrometry and hyphenated instruments in food analysis. In:Handbook of Food Analysis Instruments. S. Ötles (Ed.), ISBN: 9781420045666, CRC Press, Taylor & Francis Group (2008) pp. 197–228.

(2) J. Hajslova, T. Cajka: Gas chromatography in food analysis. In: Handbook of Food Analysis Instruments. S. Ötles (Ed.), ISBN: 9781420045666, CRC Press, Taylor & Francis Group (2008) pp. 119–144.

(1) J. Hajšlová, T. Cajka: Gas chromatography-mass spectrometry (GC-MS). In: Food Toxicants Analysis. Y. Picó (Ed.), ISBN: 978-0-444-52843-8, Elsevier, Oxford, UK (2006) pp. 419–473. (doi: 10.1016/B978-044452843-8/50013-4)